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Synthesis of Silver Nanoparticles Using Beijing Grass Extract as Reducing Agent and The Comparative Study of AgNPs Toxicity
1Department of Applied Sciences, Universiti Teknologi MARA, Cawangan Pulau Pinang, Permatang Pauh, PULAU PINANG, Malaysia
2School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, Cawangan Pulau Pinang, Permatang Pauh, PULAU PINANG, Malaysia
3Centre for Research in Biotechnology for Agriculture, Universiti Malaya, Kuala Lumpur, KUALA LUMPUR, Malaysia
*Email Address : ainorkhilah_sp@uitm.edu.my
Abstract : The popularity of AgNPs has increased in a variety of fields due to their unique properties in terms of optical, electrical, and antibacterial properties. Thus, study on the formation and toxicity of AgNPs are critical. In this study, AgNPs were prepared via a biological method utilizing Beijing grass extract (BGE) as the reducing agent and silver nirate as the metal precursor, conducted at room temperature (22±3 oC). The effects of different concentrations of reducing agent and volume ratio of metal precursor to reducing agent were investigated in this study. The formation of AgNPs were observed by UV-vis spectrophotometer based on their wavelength and absorption band. The synthesized AgNPs using optimum conditions were further characterized by FTIR and TEM. It was found that the AgNPs were characterized with spherical shape with particle size range between 1 nm and 8 nm. The FTIR spectra confirmed the formation of AgNPs capped with plant constituents of BGE. Comparative study on the toxicity effect of AgNPs on human cells and marine invertebrates were also discussed in this study. From this study, Beijing grass was proven effective as a reducing agent for synthesizing small-sized AgNPs with high dispersion.
Keywords : Silver nanoparticles, Silver nitrate, Beijing grass extract, Toxicity, UV-Vis